phase bacteria suspension Search Results


mixture of two formaldehyde fixed strains p aeruginosa pao1  (ATCC)
99
ATCC mixture of two formaldehyde fixed strains p aeruginosa pao1
Studies concerning the production and application of IgY antibodies specific to P. <t> aeruginosa </t> .
Mixture Of Two Formaldehyde Fixed Strains P Aeruginosa Pao1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cobra venom factor cvf  (Quidel)
95
Quidel cobra venom factor cvf
FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with <t>5-mg</t> <t>17-estradiol</t> slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with <t>CVF</t> to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.
Cobra Venom Factor Cvf, supplied by Quidel, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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thin-layer mueller-hinton agar  (Becton Dickinson)
90
Becton Dickinson thin-layer mueller-hinton agar
FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with <t>5-mg</t> <t>17-estradiol</t> slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with <t>CVF</t> to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.
Thin Layer Mueller Hinton Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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log-phase bioluminescent s. aureus (xen29, caliper lifesciences bioware)  (BioWare Corporation)
90
BioWare Corporation log-phase bioluminescent s. aureus (xen29, caliper lifesciences bioware)
FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with <t>5-mg</t> <t>17-estradiol</t> slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with <t>CVF</t> to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.
Log Phase Bioluminescent S. Aureus (Xen29, Caliper Lifesciences Bioware), supplied by BioWare Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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medium log phase bacterial culture suspension  (ATCC)
96
ATCC medium log phase bacterial culture suspension
FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with <t>5-mg</t> <t>17-estradiol</t> slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with <t>CVF</t> to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.
Medium Log Phase Bacterial Culture Suspension, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phase contrast microscope  (Olympus)
99
Olympus phase contrast microscope
FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with <t>5-mg</t> <t>17-estradiol</t> slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with <t>CVF</t> to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.
Phase Contrast Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s aureus atcc 25923  (ATCC)
99
ATCC s aureus atcc 25923
FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with <t>5-mg</t> <t>17-estradiol</t> slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with <t>CVF</t> to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.
S Aureus Atcc 25923, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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suspension  (ATCC)
99
ATCC suspension
FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with <t>5-mg</t> <t>17-estradiol</t> slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with <t>CVF</t> to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.
Suspension, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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suspension - by Bioz Stars, 2026-05
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sterile black polycarbonate filter membranes  (GE Healthcare)
99
GE Healthcare sterile black polycarbonate filter membranes
FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with <t>5-mg</t> <t>17-estradiol</t> slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with <t>CVF</t> to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.
Sterile Black Polycarbonate Filter Membranes, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mid logarithmic phase e coli atcc 4157  (ATCC)
95
ATCC mid logarithmic phase e coli atcc 4157
FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with <t>5-mg</t> <t>17-estradiol</t> slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with <t>CVF</t> to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.
Mid Logarithmic Phase E Coli Atcc 4157, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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middlebrook 7h9 powder  (MiddleBrook Pharmaceuticals)
90
MiddleBrook Pharmaceuticals middlebrook 7h9 powder
FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with <t>5-mg</t> <t>17-estradiol</t> slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with <t>CVF</t> to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.
Middlebrook 7h9 Powder, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sterile water  (Bio-Rad)
88
Bio-Rad sterile water
FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with <t>5-mg</t> <t>17-estradiol</t> slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with <t>CVF</t> to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.
Sterile Water, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Studies concerning the production and application of IgY antibodies specific to P. aeruginosa .

Journal: Frontiers in Immunology

Article Title: IgYs: on her majesty’s secret service

doi: 10.3389/fimmu.2023.1199427

Figure Lengend Snippet: Studies concerning the production and application of IgY antibodies specific to P. aeruginosa .

Article Snippet: Mixture of two formaldehyde-fixed strains P. aeruginosa : PAO1 (ATCC 15692) and Habs1 (ATCC 33348) , Phase I clinical trial; gargling prevention against chronic P. aeruginosa infection , Kollberg et al. ( ) .

Techniques: Activity Assay, In Vitro, Western Blot, Adjuvant, Growth Inhibition Assay, Infection, Concentration Assay, Animal Model, Inhibition, Incubation, Bacteria, Recombinant, Membrane

Hyperimmune IgYs—immunogens preparation and application of products.

Journal: Frontiers in Immunology

Article Title: IgYs: on her majesty’s secret service

doi: 10.3389/fimmu.2023.1199427

Figure Lengend Snippet: Hyperimmune IgYs—immunogens preparation and application of products.

Article Snippet: Mixture of two formaldehyde-fixed strains P. aeruginosa : PAO1 (ATCC 15692) and Habs1 (ATCC 33348) , Phase I clinical trial; gargling prevention against chronic P. aeruginosa infection , Kollberg et al. ( ) .

Techniques: Bacteria, Adjuvant, Inhibition, Suspension, Disruption, Cream, Activity Assay, Foaming

Commercially available products intended for human use.

Journal: Frontiers in Immunology

Article Title: IgYs: on her majesty’s secret service

doi: 10.3389/fimmu.2023.1199427

Figure Lengend Snippet: Commercially available products intended for human use.

Article Snippet: Mixture of two formaldehyde-fixed strains P. aeruginosa : PAO1 (ATCC 15692) and Habs1 (ATCC 33348) , Phase I clinical trial; gargling prevention against chronic P. aeruginosa infection , Kollberg et al. ( ) .

Techniques: Activity Assay, Bacteria, Recombinant

FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with 5-mg 17-estradiol slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with CVF to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.

Journal: Infection and Immunity

Article Title: Opacity Proteins Increase Neisseria gonorrhoeae Fitness in the Female Genital Tract Due to a Factor under Ovarian Control

doi: 10.1128/iai.00996-09

Figure Lengend Snippet: FIG. 6. Fluctuations in vaginal C3 levels occur in uninfected mice, but C3 depletion does not abrogate the early selection for Opa variants. (A) Concentration of C3 in vaginal washes from uninfected mice that were treated with 5-mg 17-estradiol slow-release pellets. A significantly higher concentration of C3 was present in vaginal washes from days 7 and 8 (late phase) than in vaginal washes from days 3 to 5 (middle phase). The results of two independent experiments in which 10 to 13 mice per day were used were combined. (B) Recovery of Opa variants in mice treated with CVF to deplete C3 the day prior to inoculation with wild-type bacteria. Control mice were treated with PBS. Mice were challenged with a predominantly Opa (80%) suspension of wild-type FA1090 gonococci, and the proportion of Opa variant colonies among colonies isolated from vaginal mucus was determined on days 1 to 3 postinoculation. Selection for Opa expression, as defined as a 2-fold increase compared to the inoculum (dotted line), occurred in both groups on day 1 or day 2 after bacterial challenge. The results of a single experiment in which there were 3 or 4 mice per group are shown. (C) Effect of C3 depletion on the recovery of strain FA1090opaA-K. There was no difference in the average numbers of Opa-deficient gonococci recovered from mice treated with CFU (solid line) and mice treated with PBS (dashed line) on days 1 to 3 of infection. The results are the results of a single experiment performed with 7 mice per group, and similar results were obtained in a second independent experiment. In all panels, the error bars indicate the standard errors of the means.

Article Snippet: To study the effect of complement on the early phase of infection, cobra venom factor (CVF) (Quidel) was administered intraperitoneally to groups of estradiol-treated mice (20 g CVF per 18- to 21-g mouse) to deplete C3 the day before bacterial inoculation.

Techniques: Selection, Concentration Assay, Bacteria, Control, Suspension, Variant Assay, Isolation, Expressing, Infection